Dionex As, Operator'S Manual

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B • Installation
Doc. 065051-03 1/08
215
6. If the sample preparation option is installed, follow these steps to
prime the lines from each installed reagent reservoir (see
Figure B-49 ):
a. Select the reagent reservoir, enter 2000 μ L in the Prime Volume
field, and press Prime .
b. Repeat Step 6 for each additional reagent reservoir.
7. When priming is complete, there are often small air bubbles in the top
area of the syringe. Remove the bubbles before proceeding. See
Section B.3.14 for instructions.
Suggested Priming Volumes
Sample Syringe Size Suggested Priming Volume
≤
1,000
μ
L 2,000 to 2,500
μ
L
5,000
μ
L
9,999
μ
L
a
a. The maximum value that can be entered.
10,000
μ
L
19,998
μ
L
b
b. Run two priming cycles of 9,999
μ
L each.
Table B-2. Suggested Priming Volumes
(Sample Preparation Option Not Installed)
Reservoir Prep Syringe Size Suggested Priming Volume
Flush 250
μ
L
2,000
μ
L
500
μ
L
2,000
μ
L
1,000
μ
L
4,000
μ
L
2.5 mL
5,000
μ
L
5 mL
9,999
μ
L
a
a. The maximum value that can be entered.
10 mL
19,998
μ
L
b
b. Run two priming cycles of 9,999
μ
L each.
A, B, C, and D
c
c. Repeat the priming procedure for each reagent reservoir.
All 2,000
μ
L
Table B-3. Suggested Priming Volumes
(Sample Preparation Option Installed)