Dionex As, Operator'S Manual

Page: 146 / 356

AS Autosampler Operator’s Manual
134
Doc. 065051-03 1/08
5.3.2 Filling the New Syringe and Removing Bubbles
Method A
1. Fill a container with isopropyl alcohol. Place the threaded end of the
syringe into the container and slowly pull liquid into the syringe.
2. Remove the syringe from the liquid and point the threaded end up
into the air. If air bubbles are present, push the syringe plunger up and
pull down rapidly with short movements to dislodge the bubbles.
Repeat until all bubbles are dislodged.
3. Place the threaded end of the syringe into the isopropyl alcohol again
and draw a full syringe volume up by slowly pulling out the plunger.
4. Keep the syringe in the liquid and slowly push the syringe plunger
until all bubbles exit the syringe. Then, fill the syringe completely.
5. Remove the syringe from the liquid. While holding it vertically, push
a small amount of liquid out the top to make sure no air remains.
Method B
1. Fill a container with isopropyl alcohol. Place the threaded end of the
syringe into the container and slowly pull liquid into the syringe.
2. Remove the syringe from the liquid and hold it with the threaded end
up under a stream of deionized water.
3. Push the syringe plunger up and pull down until all bubbles are
removed.
5.3.3 Connecting the New Syringe and Flushing
1. To position the new syringe under the valve, you may need to lower
move the syringe pump drive. To do this, use the
LIQUID CONTROL
screen (see Section C.9.5 ) to manually aspirate 100 to 1,000
μ
L.
2. Position the syringe under the valve.
3. Holding the syringe at the top and bottom, screw it back into the valve
and tighten fingertight.
4. Align the syringe piston with the connecting screw mount on the
syringe drive.