Dionex As, Руководство оператора

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AS Autosampler Operator’s Manual
48
Doc. 065051-03 1/08
Filling Well Plates
1. Put on powder-free latex gloves before handling well plates and
covers to minimize sample contamination.
2. Rinse the well plates and covers thoroughly with deionized water
before use to remove any contaminants.
3. Dry the well plates completely to prevent any changes in sample
concentration. You can use 690 kPa (100 psi) nitrogen gas to assist in
drying.
4. Use a pipet to fill the wells in the well plate. In general, pipet the
amount of sample to be aspirated plus 70
μ
L. See Section 3.6.4 for
details about the sample volumes aspirated for each type of injection.
The table below lists the maximum fluid volumes for each well size
and the maximum sample volumes available when the well is filled:
NOTE Changing the needle height is not recommended
when sampling from a micro-well well plate,
because the well depth is shallow. You can adjust the
needle height in a deep-well well plate as required,
provided the needle remains submerged in sample.
This ensures accurate sample aspiration.
5. Place a cover over the well plate and firmly press the indentations on
the cover into each well. This creates a tight seal and prevents sample
evaporation.
NOTE Replace covers after each use to prevent
contamination and sample evaporation. Well plates
can be reused.
Well Plate Type Maximum Volume
per well (
μ
L)
Sample Available
(
μ
L)
a
a. Assumes the well contains the maximum volume and the needle
height is 2 mm (the default).
0.45 mL micro-well with
conical (V-shaped) wells 320 250
0.5 mL micro-well with
round (U-shaped) wells 320 250
2.0 mL deep-well 1800 1700