Dionex As, Руководство оператора

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3 • Operation and Maintenance
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Setting Up a Partial-Loop, Limited-Sample Injection
1. On the
SYSTEM PARAMETERS
screen (see Section C.6.5 ), select
NORMAL
in the
SAMPLE MODE
field.
2. In the Chromeleon or Chromeleon Xpress sequence (see Figure 3-19 )
or in the front panel schedule (see Section D.3 ), enter an injection
volume that is less than the volume of the sample loop installed on the
injection valve.
3. In the Chromeleon or Chromeleon Xpress program (see Figure 3-21 ),
or on the
SYSTEM PARAMETERS
screen, enter a cut volume of zero.
Example Partial-Loop, Limited-Sample Injection Parameters :
Sample loop size: 100 μ L
Injection volume: 50
μ
L
Cut volume: 0 μ L
To perform the injection, the AS aspirates 50 μ L from the sample vial
(the inject volume) and then enough flush fluid is drawn into the
syringe to position the sample. The 50
μ
L of sample is positioned just
before the loop and is then loaded onto the loop and injected.