Dionex As, Руководство оператора

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3 • Operation and Maintenance
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Full-Loop Sample Volume Examples
For full-loop injections, if the injection volume is more than 4 times the
loop size, the full injection volume is aspirated. The excess aspirated
sample is flushed through the loop, but the amount injected is equal to the
loop size.
Large-Loop Sample Volume Examples
For large-loop injections, if the injection volume exceeds the loop size +
500
μ l, the full injection volume is aspirated. The excess sample aspirated
is flushed through the loop, but the amount actually injected is equal to
the loop size.
Installed Loop
Size (
μ
L)
Injection
Volume from
Chromeleon
Sequence (
μ
L)
Sample
Aspirated from
Vial or Well
(
μ
L)
a
a. Excess aspirated sample is used to flush the loop before and after injection.
Sample Volume
Injected onto
Column (
μ
L)
10 10 40 10
10 35 40 10
10 60 60 10
25 25 100 25
25 75 100 25
25 150 150 25
Table 3-6. Full-Loop Sample Volume Examples
Installed Loop
Size (
μ
L)
Injection
Volume from
Chromeleon
Sequence (
μ
L)
Sample
Aspirated from
Vial or Well
(
μ
L)
a
a. Excess aspirated sample is used to flush the loop before and after injection.
Sample Volume
Injected onto
Column (
μ
L)
500 500 1,000 500
500 700 1,000 500
500 1,100 1,100 500
Table 3-7. Large-Loop Sample Volume Examples