Molecular Devices ImageXpress Confocal HT.ai, User Manual

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Transmitted Light Option
The ImageXpress Transmitted Light option is designed for brightfield and phase-contrast
transmitted-light imaging. In general terms, transmitted light is used to describe microscopy in
which light is transmitted from a source on one side of the specimen, with the objective on the
other side. Typically, the light is first passed through a condenser so high illumination is
focused on the specimen. For more information on phase contrast microscopy, visit the Nikon
website:
www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html
This section describes the following:
Transmitted Light Hardware, see below
Transmitted Light Software Configuration, see page 165
Setting Up Koehler Illumination, see page 167
Transmitted Light Phase Ring Alignment, see page 170
Performing Transmitted Light Experiments, see page 176
Replacing the Transmitted Light Bulb, see page 177
Transmitted Light Hardware
The ImageXpress Transmitted Light option consists of the following hardware components.
Nikon phase optics. Allows either phase contrast or brightfield imaging. See and Figure 7-
36 .
Phase contrast objectives. Must be selected for phase contrast imaging.
White light lamp (halogen). The software controls the white light lamp (halogen) power.
The lamp bulb has a limited life and can be replaced. See
Replacing the Transmitted Light
Bulb on page 177 .
WARNING!
The surface of the halogen lamp gets very hot during operation and
remains hot for up to 20 minutes after operation. Do not to touch the lamp surface
while it is hot.
Transmitted Light shutter. Controlled by the software. The shutter protects the sample
from the transmitted light when not imaging. The shutter window maintains the
environment of the sample. See
Figure 7-37 .
Phase alignment lens. Located in the emission filter wheel and is placed in position using
the MetaXpress software for the ImageXpress Confocal HT.ai system.
Hinge. Allows robotic access to the plate if necessary. See
Figure 7-38 .
ImageXpress Systems Power and Options Controller. Used to turn the option on and off
and control the illumination intensity using the MetaXpress software. It is shared with the
ImageXpress Environmental Control option when both options are installed.