Molecular Devices ImageXpress Confocal HT.ai, User Manual

Page: 167 / 256

Chapter 7: Expansion Solution Options
5084773 A 167
Setting Up Koehler Illumination
To ensure high-quality illumination with brightfield or phase contrast imaging, set up the
Transmitted Light option for Koehler illumination. Do this alignment anytime there is concern
about the quality of the transmitted light images or when changing plate type or slide type.
Since the objectives are parfocal, repeating this procedure after switching objectives is
unnecessary.
Figure 7-43: Transmitted Light Overview
Item Description
1 Field Aperture Shutter (O = open; C = closed)
2 Field Aperture Diaphragm Control
3 Condenser Focus Control
4 Condenser Adjustment Screws (Aperture Lateral Adjustment Control)
5 Condenser Turret
6 Field Stop Slider (Condenser Aperture Diaphragm Control)
Table 7-9: Transmitted Light Overview Description
Note: For the ImageXpress Confocal HT.ai system, make sure that the instrument is in
widefield mode before setting up Koehler illumination.