Molecular Devices ImageXpress Confocal HT.ai, User Manual

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Installing Objectives
The objectives in the ImageXpress Confocal HT.ai system are mounted in a four-position linear
selector. Objectives are very delicate components. Use special care when you handle
objectives. Also see
Compatible Objectives on page 243 .
To determine the correct position for the objective, see Correct Objective Placement on page
210 .
CAUTION! You must contact Molecular Devices Technical Support to schedule a Field
Service Engineer to install or remove a water immersion objective.
Before you replace an objective, read and follow the Maintenance Precautions on page 189 .
CAUTION! To prevent skin oils from damaging the optical coatings, we recommend
wearing powder-free gloves during the following procedure.
To install an objective:
1. Start the MetaXpress software.
2. In the MetaXpress software, move the objective changer to the position to install the
objective.
Select position 1 (the position on the right) to move the objectives to the left.
Select position 4 (the position on the left) to move the objectives to the right.
3. Exit the MetaXpress software.
4. On the ImageXpress Systems Power and Options Controller, power OFF the instrument.
5. Place the new objective in its protective case on a clean work-area surface near the front
of the ImageXpress Confocal HT.ai system.
6. Open the upper hinged side panel door of the instrument.
WARNING! PINCH HAZARD.
When you install an objective, beware of the free-
moving stage. It slides around loosely when the instrument is powered off.
CAUTION! Use caution to prevent damage when holding the objective.
7. Reach in and unscrew the objective to remove.
To access position 2, you need to remove the objective in position 1.
To access position 3, you need to remove the objective in position 4.
8. Set the correction collar on the new objective, if applicable.
9. With the objective in your hand, reach into the instrument and screw the objective into its
position.
10. Close the upper hinged side panel door of the instrument.
11. On the ImageXpress Systems Power and Options Controller, power ON the instrument.
12. Start the Meta Imaging Series Administrator Software.
13. Create a new hardware profile. See Creating Hardware Profiles on page 198 .
14. Create a new group and select the new hardware profile. See Creating an Offline Version
of the MetaXpress Software on page 129 .
15. Create a new group icon. See Creating MetaXpress Software Group Icons and Adding
Them to the Windows Desktop on page 130 .
16. Exit the Meta Imaging Series Administrator Software.
17. Start the MetaXpress software using the new group icon.