Molecular Devices ImageXpress Confocal HT.ai, User Manual

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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4. In the X and Y fields, enter the calibration values for the new objective. X and Y values
must match.
You can use the following estimated values for ImageXpress Confocal HT.ai system
calibration settings:
Note: To measure pixel sizes more accurately, in the IX Taskbar, click System
Maintenance and then click Measure Pixel Sizes. If the IX Taskbar is not installed,
contact Molecular Devices Technical Support. See
Obtaining Support on page 237 .
Objective
Magnification Unbinned
Pixel Size (um)
Width (mm) Height (mm) Area (mm
2
)
1x 6.8313 13.9905 13.9905 195.7342
2x 3.4157 6.9953 6.9953 48.9335
4x 1.7078 3.4976 3.4976 12.2334
10x 0.6831 1.3991 1.3991 1.9573
20x 0.3416 0.6995 0.6995 0.4893
40x 0.1708 0.3498 0.3498 0.1223
60x 0.1139 0.2332 0.2332 0.0544
100x 0.0683 0.1399 0.1399 0.0196
Table 8-5: Estimated Calibration Settings
5. Click Magnification drop-down and select the new objective.
6. Click the Camera drop-down and select a camera.
7. Click the Channel drop-down and select a channel.
8. Click Save to File and save the file.
9. Click Close.
See Updating Magnification Settings on page 230 as needed.