Molecular Devices ImageXpress Confocal HT.ai, User Manual

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Chapter 5: Configuring Plate Acquisition Options
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5. Configure the Image-Based Focusing options. Image-Based Focusing is available when
you select the Enable Image-Based Focusing check box on the Acquisition tab.
Option Description
Algorithm Select one of the following focusing algorithm options:
Standard: Based on a standard group of settings including a normal camera
signal level.
Low Signal: Based on a set of values designed to compensate for a low
signal level on the camera, and pixel intensities that are brighter when
slightly out of focus.
Binning Set the binning used by the camera during Auto Focus and Show Live.
Horizontal and vertical binning are always set to the same value.
Custom
Exposure
Times For individual wavelengths during autofocus. If you do not select this option,
then the software calculates the exposure time based on autofocus binning and
acquisition exposure time. If you select this option and you select either or both
Laser and Image or Laser with Image Recovery for the first acquisition
wavelength (W1), or Image-Based for any subsequent acquisition wavelengths
(W2, W3, and so on), then you must also specify values for Exposure and Gain on
the Wavelength tabs. See
Specifying the Number of Acquisition Wavelengths on
page 95 .
Allow Image-
Based
Focusing for
Recovery
from Laser-
Based Well
Bottom
Failures Enabled only if you select both Enable Laser-Based Focusing and Enable Image-
Based Focusing.
Use Image-Based Focusing only if Laser-Based Focusing cannot find the plate or
well bottom.
The image-based recovery search is centered on an estimated well bottom
offset position.
The software calculates the estimated position by using the plate bottom
position that was found during the last successful laser autofocus attempt and
adds the plate bottom thickness and post laser offset values to this plate bottom
thickness value.
Table 5-5: Autofocus Tab, Image-Based Focusing Options