Molecular Devices ImageXpress Confocal HT.ai, User Manual

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Chapter 5: Configuring Plate Acquisition Options
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Using Legacy Correction Image Shading
Note: This section is applicable if you select Legacy Correction as the shading
correction method for an acquisition wavelength. See
Configuring Acquisition
Wavelengths on page 96 .
Before you do legacy shading correction, acquire the images for each acquisition wavelength
and save the images to a stored correction images directory. The default location is
C:\Shading Images folder. See
Verifying Shading Correction Files-Legacy on page 52 .
The file name for a shading correction image must include exact matches for the Magnification,
Acquisition Mode, and Illumination settings, for example,
Shading_10XPlan Fluor_widefield_
DAPI.tif
. See Updating Legacy Shading Correction Settings on page 233 .
If you acquire an image with the full field of view, you can use the Custom Field of View feature
on the Plate tab with shading correction. See
Plate Acquisition Setup Dialog: Configure Tab,
Plate Tab on page 72 .
Note: If a shading correction image cannot be found for the combination of
Magnification, Acquisition Mode, and Illumination settings in the directory, no shading
correction is done for the wavelength.
To change the default directory location for shading correction images:
1. Select the Acquisition tab.
2. Click Directory for Stored Correction Images to display the Browse for Folder dialog.
3. Select the new location.
4. Click OK.