Molecular Devices ImageXpress Confocal HT.ai, User Manual

Page: 180 / 256

ImageXpress Confocal HT.ai High-Content Imaging System User Guide
180 5084773 A
Water Immersion Hardware Components
The Water Immersion option consists of the following hardware components:
Water Immersion Objectives, see below
Water Immersion Controller, see page 181
Water Immersion Objectives
CAUTION! Do not remove or change the water immersion objectives that are installed
within the instrument. A Molecular Devices Field Service Engineer must install and
remove water immersion objectives.
The Water Immersion option can include up to two of the water immersion objectives listed in
the following table.
Water
Immersion Objective Numerical
Aperture (NA) Working
Distance Correction
Collar Range
20X 0.95 0.95 mm 0.11 mm to 0.23 mm
40X 1.15 0.59 mm 0.15 mm to 0.19 mm
60X 1.2 0.28 mm 0.15 mm to 0.18 mm
Table 7-11: Water Immersion Objective Specifications
The water immersion objectives must be located in the first and/or fourth objective slots.
Water immersion objectives have correction collars that are similar to the correction collars on
air objectives. The objectives also have a cap that allows the creation of a bolus (bubble of
water) above the objective and that directs water to a drain. The drain maintains a vacuum to
draw water from the objective to a waste water bottle.
CAUTION! Do not touch the leak detection sensor, which is the brass color ring around
the top of the objective below the cap.