ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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4. If Koehler illumination has not been set up, move the field-stop slider, located just above
the A label on the condenser turret, all the way to the right.
Figure 7-48: Field Stop Slider
5. Select the diffusion filter or place some lens paper or lint-free wipes on top of the
condenser lens to serve as a diffuser. If needed, weigh them down slightly to keep them
from blowing away.
6. In the MetaXpress software, select the desired phase contrast objective.
7. Set the focus (Z position) to approximately 8000 µm.
If needed, you can adjust this later.
8. Select the Transmitted Light Illumination setting.
9. In the Configure Illumination dialog, to select the phase ring alignment lens, change the
IXConfocal Module Emission Filter to Position 8.
10. With no sample present, snap an image.
11. If necessary, adjust the illumination power level and the exposure time to obtain a visible
but not saturated image. The image of the phase ring might be off-center. This is
acceptable if most of the image is visible.
Tip: If the phase ring is not visible at all, contact Molecular Devices Technical
Support. See
.
Figure 7-49: Out of Focus Image of Phase Contrast Ring
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