Molecular Devices ImageXpress Confocal HT.ai, User Manual

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Configuring Acquisition Wavelengths
Use the W# tabs below the Wavelength tab to configure acquisition wavelengths. The
sequence in which you configure the acquisition wavelengths corresponds to the acquisition
order. For example, if you configure the W1 tab as a DAPI wavelength and then configure the
W2 tab as a FITC wavelength, then the DAPI acquisition wavelength runs first and the FITC
acquisition wavelength runs second.
To configure each individual wavelength:
1. Select the W1 tab.
2. Click the Illumination drop-down and select the illumination setting; for example, DAPI. The
selection name displays in the Active Wavelength field under the plate map and on the
individual tab.
Figure 5-18: W1 Wavelength Tab
3. Configure the remaining acquisition wavelength options, where Offset (µm) is the Z motor
offset distance for each wavelength. For the first wavelength, if you use laser-based
focusing, then the offset is the distance between the bottom of the well and the in-focus
plane. If you use just image-based focusing, then no offset is required for the first
wavelength. For the second and subsequent wavelengths, with laser-based or image-
based focusing, the offset is the difference between the focus position of the Z motor
using the first wavelength and the focus position of the Z motor using the second or
subsequent wavelength.