Molecular Devices ImageXpress Confocal HT.ai, User Manual

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Appendix B: Compatible Objectives
5084773 A 245
Objective Magnification and Type
(Molecular Devices Part Number) Phase
Contrast Numerical
Aperture (NA) Working
Distance
Plate Compatibility
60x Plan Apo Lambda Oil
(1-6300-0417) --- 1.40 0.13 mm Thin bottom (0.17 mm)
1
Thin bottom (0.17 mm) No Skirt
60x Plan Fluor ELWD ADL
cc 0.1 mm to 1.3 mm
(6500-0113) Ph2 0.70 1.8 mm to
2.62 mm Thin bottom (0.17 mm)
3
Thin bottom (0.17 mm) No Skirt
3
Thick bottom (0.25 mm to 1 mm)
60xc WI (Water Immersion) CFI Plan
Apochromat VC
cc 0.15 mm to 0.18 mm
(5075818) --- 1.2 0.28 mm Thin bottom (0.17 mm)
1
Thin bottom (0.17 mm) No Skirt
100x Plan Fluor Oil
(1-6300-0418) --- 1.30 0.2 mm Thin bottom (0.17 mm)
1
Thin bottom (0.17 mm) No Skirt
100x CFI L Plan EPI
cc 0 mm to 0.7 mm
(1-6300-0419) --- 0.85 1.2 mm to
0.85 mm Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
Table B-1: Nikon Objectives Compatible with the ImageXpress Confocal HT.ai System and Settings (continued)
1
Potential interference with plate skirt when imaging edge wells.
2
Image degradation above plate thickness 0.3 mm.
3
20x and 40x ELWD image through cover slips, but other objectives give better resolution and shorter exposures.
Note:
When imaging edge wells with thin-bottom plates, the short working distance of the following objectives
can cause interference with the plate skirt:
20X S Fluor
20X Plan Apo
20xc WI CFI Apochromat LWD Lambda S
40X S Fluor
40X Plan Apo
40X Plan Fluor Oil
40xc WI CFI Apochromat LWD Lambda S
60X Plan Fluor
60X Plan Apo Oil
60xc WI CFI Plan Apochromat VC
100X Plan Fluor Oil
If the software does not automatically block the edge wells, you should either omit the edge wells or
use a plate with a low skirt