Molecular Devices ImageXpress Confocal HT.ai, User Manual

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Chapter 5: Configuring Plate Acquisition Options
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Acquiring a Fixed Number of Sites in Each Well
Use the Sites to Visit tab to acquire a fixed number of sites in each well.
Figure 5-9: Configure Tab, Sites to Visit Tab, Fixed Number of Sites Option
To acquire a fixed number of sites in each well:
1. Select the Sites to Visit tab.
2. Select the Fixed Number of Sites Site Option.
3. Select the Custom Field of View (%) check box to specify a percentage of the field of view
for the camera to acquire, and then enter the X (width) value and Y (height) value. This is
useful to acquire images from an unevenly illuminated full field or to acquire images that
include the area outside the well boundary.
Note:
The full field of view for the camera is 100 percent (100% in the X field and 100%
in the Y field). For example, if the full field of view width is 1000 µm, then to
acquire a width of 500 µm, enter 50 in the X field.
Custom field of view is compatible with full-size shading correction images
when you use Legacy Correction. See
Using Legacy Correction Image
Shading on page 105 .