Molecular Devices ImageXpress Confocal HT.ai, User Manual

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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4. Select the Timelapse tab.
Figure 7-23: Plate Acquisition Setup Dialog, Timelapse Tab
5. In the Number of time points field, enter the number of time points.
6. Click the Perform Time Series For drop-down:
Select All Selected Wells for long time lapse experiments. The system images all
wells at the first time point, then all wells at the second time point, and so on.
Select One Well Then The Next for fast-kinetic experiments. The system images all
time points on well 1, then moves to well 2 and images all time points, and so on.
Select One Row Then The Next or One Column Then The Next for experiments that
use manual pipetting.
7. If applicable, select the Autofocus tab.
Figure 7-24: Plate Acquisition Setup Dialog, Autofocus Tab
8. Click the Timelapse Autofocus drop-down:
Select First Timepoint Only for fast-kinetic experiments.
Select All Timepoints for long time lapse experiments.
Select Every N
th
Timepoint to autofocus regularly during a time lapse experiment.