Molecular Devices ImageXpress Confocal HT.ai, User Manual

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Chapter 5: Configuring Plate Acquisition Options
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Specifying the Number of Acquisition Wavelengths
Use the Wavelengths tab to specify the total number of acquisition wavelengths for an
experiment. You must specify at least one acquisition wavelength and you can specify up to
eight. When you specify more than one wavelength, subtabs display below the Wavelength
tab where you configure exposure time, autofocus, and time lapse settings for each acquisition
wavelength. Red indicates that you have yet to configure the acquisition wavelengths.
Figure 5-17: Wavelength Tab with Three Wavelengths
Tip: As you configure an acquisition protocol, you can open the Summary panel to view
the current values for the protocol settings. See
Viewing the Summary Panel on page
123 .
To specify the number of acquisition wavelengths, do the following:
1. Select the Wavelengths tab.
2. In the Number of Wavelengths field, specify the total number of wavelengths to be used
for acquisition, where the minimum value is one and the maximum value is eight.
3. In the TL Legacy Shading Correction Refinement Level field, enter the refinement level.
The software applies this option if you select Autocorrect for TL Legacy for Shading
Correction for one or more acquisition wavelengths. The larger the value for the
refinement level, the flatter the transmitted light image will be. Increasing this value slows
the acquisition routine. The single value that you enter applies to each acquisition
wavelength for which you select Auto Correction for TL Legacy as the shading correction
method. See
W# Tab: Shading Correction Options on page 102 .
4. Select the individual W# subtabs to configure each acquisition wavelength. See
Configuring Acquisition Wavelengths on page 96 .