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Appendix B: Compatible Objectives
The following table details the Nikon objectives that are compatible with the ImageXpress Confocal HT.ai
system. It also provides plate compatibility information and theoretical calculation of confocal performance for
the objectives you use in the system.
Objective Magnification and Type
(Molecular Devices Part Number)
Phase
Contrast
Numerical
Aperture (NA)
Working
Distance
Plate Compatibility
1x Plan Achromat
(650-0119)
---
0.04
3.2 mm
Thin bottom (0.17 mm)
1
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
1
2x Plan Apo Lambda
(1-6300-0451)
---
0.10
8.5 mm
Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
4x S Fluor
(1-6300-0189)
---
0.20
15.5 mm
Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
4x Plan Apo Lambda
(1-6300-0121)
---
0.20
20 mm
Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
4x Plan Fluor DL
(1-6300-0292)
PhL
0.13
16.2 mm
Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
10x S Fluor
(1-6300-0122)
---
0.50
1.2 mm
Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
2
10x Plan Apo Lambda
(6500-0120)
---
0.45
4.0 mm
Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
10x Plan Fluor DLL
(1-6300-0294)
Ph1
0.30
16.0 mm
Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
10x Plan Fluor DL
(1-6300-0293)
Ph1
0.30
15.2 mm
Thin bottom (0.17 mm)
Thin bottom (0.17 mm) No Skirt
Thick bottom (0.25 mm to 1 mm)
20x Super Plan Fluor ELWD
cc 0 mm to 2 mm
(6500-0108)
---
0.45
8.1 mm to
7.0 mm
Thin bottom (0.17 mm)
3
Thin bottom (0.17 mm) No Skirt
3
Thick bottom (0.25 mm to 1 mm)
20x S Fluor
(1-6300-0411)
---
0.75
1.0 mm
Thin bottom (0.17 mm)
1
Thin bottom (0.17 mm) No Skirt
Table B-1: Nikon Objectives Compatible with the ImageXpress Confocal HT.ai System and Settings
B
Summary of Contents for ImageXpress Confocal HT.ai
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