Molecular Devices ImageXpress Confocal HT.ai, User Manual

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ImageXpress Confocal HT.ai High-Content Imaging System User Guide
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Performing Transmitted Light Experiments
Do the following procedure if you are running an experiment with Transmitted Light as one of
the selected wavelengths.
To run a Transmitted Light experiment:
1. If you run a phase-contrast experiment, make sure to select a phase-contrast objective.
Phase-contrast objectives are not required for brightfield experiments.
2. Make sure the annulus on the Transmitted Light arm matches the objective, and set it to A
for brightfield experiments.
3. Load a suitable plate. The plate must be unlidded, have a clear lid, or have a clear plate
seal on the top. Fill wells at least half-way full of liquid.
CAUTION! Condensation on a lid or plate seal can negatively affect image quality.
4. In the Configure Illumination dialog, select an Illumination setting to turn on the Transmitted
Lamp.
5. In the MetaXpress software, do one of the following to display the Plate Acquisition Setup
dialog:
From the simplified menu, click Screening > Acquisition Setup.
From the standard menu, click Screening > Plate Acquisition Setup.
6. Define one of the wavelengths as Transmitted Light.
7. Configure the remaining settings as usual.
8. Run the acquisition.
Tip: When the transmitted light unit is not in use, in the Configure Illumination dialog,
select the Transmitted Lamp Off setting to increase the life of the halogen lamp.