Bone Marrow Processing (BMP) Procedures
194
Spectra Optia Apheresis System Operator’s Manual
Functional Description of a Bone Marrow Processing (BMP)
Procedure
The BMP procedure is intended for bone marrow processing for the purpose of hematopoietic reconstitution.
During a BMP procedure, the system pumps bone marrow from the bone marrow processing (BMP) bag into the
tubing set channel and spins the centrifuge at the speed required to target the optimal (default) packing factor of
20. The AIM system adjusts the flow rate of the plasma pump to control the concentration of cells that flow
through the collect port (based on the collection preference). The BMP bag must contain at least 125 mL of RBC
for the system to establish an interface that is high enough to collect cells. When the AIM system detects cells in the
collect port, the system sounds a tone, the collect valve moves into the collect position, and the collect pump
pumps the cells into the collection bag. The plasma pump pumps plasma out of the channel and either into the
reservoir or into the plasma bag, if plasma collection is targeted. The red blood cells (RBC) are pushed out of the
channel and into the reservoir where they combine with the plasma for return to the BMP bag for additional
processing.
Preparing to Perform a Procedure
Supplies
Assemble the supplies listed on page 62 before you begin a procedure. You will also need the following additional
supplies:
•
Spectra Optia
®
BMP Accessory Set
Optional supplies:
•
Blood administration set with a male luer connector and the appropriate size filter for filtering clots, bone
chips, and debris from the bone marrow
•
Sterile tubing welder with the appropriate replacement wafers
Filtering the Bone Marrow
Filter the bone marrow according to your facility’s standard operating procedure. The bone marrow must be filtered
to remove fat, bone particles, and cellular debris before it is processed. The 200-micron filter on the administration
line in the BMP Accessory Set may be used for additional filtration, if desired.
Adding ACD-A to the Bone Marrow
The bone marrow must be adequately anticoagulated before processing to prevent clumping in the tubing set. The
recommended ratio of bone marrow to ACD-A is 10:1. Perform the following steps to calculate the necessary
volume of ACD-A and to add it to the bone marrow:
1
Determine the tare weight of the bag containing the bone marrow.
2
Weigh the bag of bone marrow (BM bag).
3
Calculate the bone marrow (BM) volume:
BM bag weight bag tare weight
–
1.058
--------------------------------------------------------------------------------
BM volume (mL)
=
SpectraOptiaOpsManual.book Page 194 Thursday, May 17, 2018 11:24 AM
