Granulocyte (PMN) Collection Procedures
162
Spectra Optia Apheresis System Operator’s Manual
Functional Description of a Granulocyte (PMN) Collection Procedure
During a PMN collection procedure, the system pumps the patient’s blood into the tubing set and spins the
centrifuge at the speed required to target the optimal (default) packing factor: 1.6, when HES is used, or 4.5, when
ACD-A is used. The AIM system adjusts the flow rate of the plasma pump to control the concentration of cells that
flow through the collect port (based on the collection preference). When the AIM system detects cells in the collect
port, the system sounds a tone, the collect valve moves into the collect position, and the collect pump pumps
granulocytes into the collection bag. The plasma pump pumps plasma out of the channel and either into the
reservoir or into the plasma bag, if plasma collection is targeted. The system always prioritizes collecting the PMN
over collecting the plasma. The red blood cells (RBC) are pushed out of the channel and into the reservoir, where
they combine with the plasma for return to the patient.
Preparing to Perform a Procedure
Supplies
Assemble the supplies listed on page 62 before you begin a procedure.
Anticoagulant Solutions for PMN Collection Procedures
You can use a solution that contains hydroxyethyl starch (HES) or ACD-A to perform a PMN collection procedure
on the Spectra Optia system.
Using HES
HES is a sedimenting agent that causes RBC to separate more efficiently from granulocytes during a PMN
collection procedure. If you choose to use HES, you must select
Yes
for HES on the PMN configuration screen or
on the options screen. “HES: Yes” appears on the main run screen as a reminder of your selection. When using
HES, you must add trisodium citrate to the HES for use as the anticoagulant. The default packing factor when
HES is used is 1.6.
Preparing an anticoagulant solution containing HES and trisodium citrate
To prepare the correct anticoagulant solution, add 30 mL to 40 mL of 46.7% trisodium citrate solution to 500 mL
of HES. To prepare an anticoagulant solution with the same concentration as ACD-A, add 38 mL of trisodium
citrate solution to the HES.
Using ACD-A
If you choose to use ACD-A, you must select
No
for HES on the PMN configuration screen or on the options
screen. “HES: No” appears on the main run screen as a reminder of your selection. Also refer to “Optimizing a
PMN Collection Procedure When Using ACD-A” on page 173 for information about adjustments you can make
to optimize the run. The default packing factor when ACD-A is used is 4.5.
Caution:
If hydroxyethyl starch (HES) is not used during a PMN collection procedure, the collection efficiency
will be lower.
SpectraOptiaOpsManual.book Page 162 Thursday, May 17, 2018 11:24 AM
