Bone Marrow Processing (BMP) Procedures
204
Spectra Optia Apheresis System Operator’s Manual
Optimizing the Run
Optimizing the Collection Preference
The collection preference controls the concentration of cells that flow through the collect port. You can increase or
decrease the collection preference during the run to accommodate the condition of the bone marrow and to achieve
your procedure goals. Consider the following guidelines when changing the collection preference:
•
Decrease the collection preference to collect as many target cells as possible. This may result in greater RBC
contamination of the collected product.
•
Increase the collection preference to minimize RBC contamination of the collected product. This may result
in a lower yield of target cells.
Monitoring the contents in the collect line and adjusting the color
Monitor the collect line where it exits the centrifuge below the cassette, and use the collection preference to adjust
the color of the contents in the line.
The Collection Preference Tool can help you evaluate which color is appropriate for the procedure. If the contents
in the line look either too light or too dark, consider changing the collection preference according to the following
guidelines:
•
If the color is too light, decrease the collection preference. This increases the concentration of cells that flow
through the collect port.
•
If the color is too dark, increase the collection preference. This decreases the concentration of cells that flow
through the collect port.
Addressing Clumping in the Connector
Clumping can affect collection efficiency by interfering with the separation in the connector. Clumping and the
formation of clots can occur during a BMP procedure if the bone marrow was not adequately anticoagulated using
ACD-A. If you see clumping in the connector, verify that the appropriate amount of ACD-A was added to the BM
bag. If the clumping persists, consider adding ACD-A to the BMP bag.
Reducing the Concentration of Platelets in the Collection Bag
Decreasing the packing factor reduces the volume of platelets flowing into the collection bag. If the concentration
of platelets in the collection bag is higher than desired, consider decreasing the packing factor to 4.5 to help reduce
the concentration.
SpectraOptiaOpsManual.book Page 204 Thursday, May 17, 2018 11:24 AM
