Mononuclear Cell (MNC) Collection Procedures
140
Spectra Optia Apheresis System Operator’s Manual
Monitoring the Run
The system displays information on the screens to help you monitor the progress of the run. To access the screens,
touch the
Run
menu button and then the tab for the screen you want to view. Be sure to verify that the volumes
displayed on the screens are consistent with the actual volumes in the fluid containers and in the bags.
See “Glossary of Terms” on page 278 for descriptions of buttons that appear on the screens.
Main Run Screen
The main run screen is the default run screen and displays or provides access to the information you most likely
need to monitor the run. If you want to return to the main run screen after viewing a different screen, touch the go
back button, the active menu button, or the tab for the current screen. There is no tab for the main run screen.
Collection Status Screen
To access the collection status screen, touch the
Collection Status
tab. Use this screen to monitor the progress of
the accumulation phase and to adjust the collection preference, if necessary. You can also find information about
the current and previous collection and accumulation phases on this screen.
Collection preference
The collection preference is a reference number that the AIM system uses to determine the concentration of cells
that should flow through the collect port. The collection preference can affect the type of cells that fill the chamber
and how quickly the chamber fills.
AIM system and the concentration of cells flowing through the collect port
The AIM system cannot identify the type of cells that flow through the collect port; it can only control the
concentration of cells. It determines the concentration by evaluating how much light penetrates the contents in the
collect port as follows:
•
High collection preference: More light can penetrate the contents. The concentration is low and the color in
the collect port looks light.
•
Low collection preference: Less light can penetrate the contents. The concentration is high and the color in
the collect port looks dark.
The system then uses this information to adjust the plasma pump flow rate and achieve the specified collection
preference.
Collection preference and cell counts
The system uses the WBC count and the platelet count that you entered on the patient data screen to calculate the
collection preference for the run as follows:
•
The higher the cell counts, the lower the collection preference.
•
The lower the cell counts, the higher the collection preference
For information about optimizing the collection preference during the run, see “Optimizing the Collection
Preference” on page 144.
Trend graph
The trend graph shows the status of the current accumulation phase. It represents an area of the buffy coat that is in
the connector. The horizontal black line on the graph indicates the current collection preference. The green
diamonds indicate the concentration of cells flowing into the chamber as compared to the collection preference.
SpectraOptiaOpsManual.book Page 140 Thursday, May 17, 2018 11:24 AM
