Mononuclear Cell (MNC) Collection Procedures
142
Spectra Optia Apheresis System Operator’s Manual
Advanced Control Screen
To access the advanced control screen, touch the
Advanced Control
tab. Use this screen to make adjustments to the
run.
Flush chamber
To immediately change the position of the collect valve and flush the chamber, perform one of the following steps:
•
To return the contents of the chamber to the patient, touch the button for return to patient and then touch
Apply
. The collect pump pumps a volume of plasma equal to 1.5 multiplied by the flush volume through the
chamber to flush the contents of the chamber to the patient. If you return the contents of the chamber to the
patient, the system does not display the inlet volume that was processed before you initiated the return on the
graph on the collection status screen.
•
To initiate a collection phase and flush the contents of the chamber into the collection bag, touch the button
for collect into bag and then touch
Apply
.
Clumping in connector
To address clumping in the connector, touch
Decrease Inlet:AC Ratio
. The system decreases the inlet:AC ratio to
8:1. After 100 mL of inlet volume has been processed, a screen appears with instructions for ensuring that the
clumping is resolved and for resuming the procedure. For additional information about anticoagulation in the
tubing set, see “Managing Anticoagulation of the Extracorporeal Circuit” on page 221.
Collection phase control
The collection phase control allows you to specify how collection phases are initiated. The selected control also
appears on the collection status screen. The following collection phase controls are available:
•
System
(default control): The system initiates a collection phase when the RBC detector detects RBC in the
collect line and the system predicts that the chamber is at least 50% full of target cells. The system uses an
algorithm to predict the number of target cells in the chamber.
•
Inlet
: The system initiates a collection phase after a specified inlet volume (mL) has been processed. When
you select this control, a data entry pad appears for you to enter the desired volume to be processed. The
volume that you enter appears on the button. When the Inlet control is selected, the test tube icon ( )
appears on the graph of the inlet volume processed on the collection status screen.
•
Operator
: This control allows the operator to initiate a collection phase when desired. To initiate a collection
phase, you must go to the advanced control screen and touch the collect into bag button. The system enters
Caution status when this control is selected. When the Operator control is selected, the operator override
icon ( ) appears on the graph of the inlet volume processed on the collection status screen.
•
RBC Detector
: The system initiates a collection phase when the RBC detector detects RBC in the collect line
and the system predicts that the chamber is at least 25% full of target cells. The system uses an algorithm to
predict the number of target cells in the chamber.
Chamber
The configured volumes for the chamber flush and the chamber chase appear on the buttons. You can adjust the
volumes at any time during the run. Any changes to the volumes take immediate effect and apply to the current
procedure only.
SpectraOptiaOpsManual.book Page 142 Thursday, May 17, 2018 11:24 AM
