Spectra Optia Apheresis System Operator’s Manual
201
Bone Marrow Processing (BMP) Procedures
14
Preparing to Start the Run
Follow the instructions below and on the screens to connect the BMP bag to the tubing set and to prime the inlet
line.
Connecting the BMP Bag to the Tubing Set
1
Lay the BMP bag lower than the saline container.
2
Connect the inlet and return lines on the BMP bag to the inlet and return lines on the tubing set using one
of the following two methods:
• Use the luer connectors on the inlet and return lines to connect the BMP bag to the tubing set. Verify that
the luer connections are secure and that the connections do not leak.
• Use a sterile tubing welder to connect the BMP bag to the tubing set. Follow the manufacturer’s
instructions for using the sterile tubing welder. Using a sterile tubing welder to connect the BMP bag helps
to maintain a functionally closed system.
3
Touch
Confirm.
Priming the Inlet Line
1
Unclamp the inlet line and prime the line. Be sure to prime the inlet line through the port on the BMP bag.
Be careful not to add more saline than is necessary to the BMP bag to prime the inlet line.
2
Close the inlet saline line only.
3
Touch
Confirm.
Starting the Run
1
Follow the instructions on the screen to hang the BMP bag and ensure that it is not creased. Be sure to hang
the BMP bag on the back hooks of the IV pole, and use each eyelet on the bag and one eyelet per hook.
Remove any creases in the BMP bag so that the cells can circulate freely in the bag.
2
Touch
Start Run
. The system begins drawing the bone marrow into the tubing set. The main run screen
appears, and the system diverts approximately 40 mL of saline used to prime the tubing set into the saline
container.
Mixing the Contents of the BMP Bag During the Run
Mixing the contents of the BMP bag helps incorporate the saline coming into the bag from the return line and
prevents the cellular components from settling in areas of the bag. Gently mix the contents several times during
each BM cycle. When mixing, leave the bag hanging on the IV pole and avoid drawing air into the inlet line.
Note:
You must hang the BMP bag on the back hooks of the IV pole. The front hooks cannot properly
accommodate the bag.
SpectraOptiaOpsManual.book Page 201 Thursday, May 17, 2018 11:24 AM
