Spectra Optia Apheresis System Operator’s Manual
195
Bone Marrow Processing (BMP) Procedures
14
4
Calculate the volume of ACD-A to add to the BM bag:
5
Add the ACD-A volume from step 4 to the BM bag and mix the contents of the bag thoroughly.
6
Calculate the total BM volume (BMV) to enter on the BM data screen during the procedure:
7
Measure the hematocrit (Hct) of the bone marrow in the BM bag. This is the Hct you enter on the BM data
screen during the procedure.
Transferring the Bone Marrow Into the BMP Accessory Set
Perform the following steps to transfer the bone marrow from the BM bag into the BMP Accessory Set:
1
Verify that the accessory set has not expired by checking the expiration date on the cover of the package.
2
Open the package containing the accessory set and unpack the contents.
3
Unfold the BMP bag and lay it on a flat surface.
4
Clamp the following lines of the accessory set:
• Inlet line (red clamp)
• Sample bulb assembly (white clamp)
• Lines to both spikes (white clamps) on the administration line
• Both lines (white clamps) of the luer connector assembly
• Return line (blue clamp)
5
Connect the BM bag to the administration line using one of the following two methods:
• Spike the BM bag using one of the spikes on the administration line. The 200–micron filter on the line to
the spike provides additional filtration.
• Connect the BM bag to the luer connector assembly on the administration line. If the bone marrow
requires filtration, use a blood administration set with the appropriate size filter to connect the bag to the
luer connector.
6
Unclamp the line to the spike or on the line of the luer connector assembly on the administration line.
7
Hang or hold the BM bag above the BMP bag and transfer the bone marrow.
8
Seal the administration line below the luer connector on the inlet line (Figure 14-1). You must seal the line
below the connector, or you will not be able to connect the BMP bag to the inlet line of the tubing set.
BM volume (mL)
10
------------------------------------------
ACD-A (mL)
=
BM volume (mL) ACD-A (mL)
BMV (mL)
=
+
SpectraOptiaOpsManual.book Page 195 Thursday, May 17, 2018 11:24 AM
