Spectra Optia Apheresis System Operator’s Manual
271
Supplementary Information
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Using the Sample Bulbs to Obtain a Product Sample
This section contains instructions for using a sample bulb on the collection bag to obtain a product sample, and
options for removing a sample from a bulb.
Obtaining a Product Sample
To obtain a product sample using the sample bulbs on the collection bag, perform the following steps:
1
Ensure that the line between the collection bag and the manifold on the sample bulb assembly is clamped.
2
Clamp one of the lines between the manifold and the sample bulb.
3
Gently mix the product in the bag to ensure that you obtain a representative sample.
4
Unclamp the line between the collection bag and the manifold on the sample bulb assembly.
5
Gently squeeze the sample bulb attached to the line that is not clamped to withdraw the desired amount of
the sample.
6
To express any excess sample back into the collection bag, perform the following steps:
a. Invert the sample bulb, and hold it above the fluid level of the collection bag.
b. Gently squeeze the sample bulb to express the excess sample into the bag.
7
To use the residual air in the sample bulb to clear the fluid from the line between the collection bag and the
sample bulb, perform the following steps:
a. Hold the sample bulb upright and below the collection bag.
b. Gently squeeze the sample bulb. The residual air in the bulb pushes the product from the line into the
collection bag.
c. While maintaining pressure on the sample bulb, clamp the line between the manifold and the sample bulb.
8
Before you remove the sample bulb containing the product sample, permanently seal the line between the
clamp below the manifold and the sample bulb.
9
Disconnect the sample bulb at the seal on the line.
Removing a Product Sample From the Sample Bulb
Below are three options for removing a sample from a sample bulb. Select one of these options or follow your own
process or standard operating procedure to remove the sample.
Option 1: Convert the sample bulb to a test tube to remove the sample
To convert the sample bulb to a test tube to remove the sample, cut off the top of the sample bulb at the dotted line
on the bulb. The bulb accommodates stoppers suitable for use with 12 mm × 75 mm test tubes.
Option 2: Pour the product sample into a test tube or other container
To pour the sample into a test tube or other container, cut the line below the seal, and gently squeeze the sample
bulb to express the sample into the container.
Option 3: Aspirate the sample from the sample bulb using a needle or a needleless adapter with an attached syringe
To aspirate the sample from the bulb, perform the following steps:
1
Insert a needle or a needleless adapter with an attached syringe into the sampling port.
2
Invert the sample bulb.
3
Slowly aspirate the product sample into the syringe. A small amount of product may remain in the sampling
port.
SpectraOptiaOpsManual.book Page 271 Thursday, May 17, 2018 11:24 AM
