Mononuclear Cell (MNC) Collection Procedures
144
Spectra Optia Apheresis System Operator’s Manual
Optimizing the Run
Optimizing the Collection Preference
The collection preference controls the concentration of cells that flow through the collect port. The system uses the
WBC count and the platelet count that you entered on the patient data screen to calculate the collection
preference. You can use the up and down arrows on the screen to increase or decrease the collection preference
during the run to accommodate the patient’s condition and to achieve your procedure goals. Consider the
following guidelines when adjusting the collection preference, keeping in mind that the patient’s WBC and platelet
counts affect the contents of the collection:
•
Use a low collection preference to increase the concentration of cells in the collect port. The system adjusts the
plasma pump flow rate so that it collects more deeply in the buffy coat. This maximizes the yield of target cells
but could increase RBC contamination of the collected product.
•
Use a high collection preference to decrease the concentration of cells in the collect port. The system adjusts
the plasma pump flow rate so that it collects more shallowly in the buffy coat. This helps to minimize RBC
contamination of the collected product but could result in a lower yield of target cells.
Minimizing Buffy Coat Accumulation
A buffy coat can accumulate in the connector for several reasons. To minimize accumulation of a buffy coat,
consider performing the following steps:
•
Avoid pausing the pumps and conditions that cause the pumps to pause, such as alarm conditions. After the
pumps restart, it takes time for the collect pump to reach the target flow rate, which slows the collection of
cells from the connector.
•
Decrease the collection preference. The interface might not be high enough to allow the system to collect the
buffy coat.
•
Decrease the inlet pump flow rate until the buffy coat stops accumulating. If the inlet pump flow rate is too
fast, cells enter the channel faster than the collect pump can remove them, causing the accumulation. The
maximum flow rate for the collect pump during the accumulation phase is 3 mL/min.
•
Confirm that the platelet count that was entered on the patient data screen is correct. If decreasing the
collection preference and the inlet pump flow rate did not reduce the accumulation and the collect pump flow
rate is less than 3 mL/min, increase the entered platelet count by 50. Do this only if you considered all other
options and you were still unable to reduce the buffy coat.
Decreasing the Run Time
Consider the following alternatives to decrease the predicted run time if a shorter run is necessary:
•
Avoid pausing the pumps and conditions that cause the pumps to pause, such as alarm conditions. After the
pumps restart, it takes time for the pumps to reach the target flow rates again, which adds time to the run.
•
Increase the inlet pump flow rate, or increase the AC infusion rate. Increasing these rates allows the system to
process more blood in less time. Be aware that either option results in faster delivery of AC to the patient,
which increases the potential for the patient to experience a citrate reaction.
SpectraOptiaOpsManual.book Page 144 Thursday, May 17, 2018 11:24 AM
