Troubleshooting
220
Spectra Optia Apheresis System Operator’s Manual
Resuming the Procedure in Semi-Automatic Mode
Once you have entered Semi-Automatic mode, follow the instructions below to resume the procedure.
Exchange procedures
1
Look through the viewport to view the interface position. The interface position should appear low and the
RBC layer should be thin.
To adjust the interface position, go to the patient data screen and change the entered Hct. If the position
seems too high, increase the Hct by 3 percentage points. You may do this up to three times for a total increase
of 9 percentage points.
2
Monitor the interface position and adjust the position again, if necessary.
MNC collection procedures
1
Go to the collection status screen.
2
Look through the view port to view the concentration of cells flowing through the collect port. Once the
interface is in the correct position, you should see swirls of cells flowing through the collect port. If the AIM
system is enabled, the image of the collect port on the screen should also show the swirls.
3
Ensure the concentration of cells flowing through the collect port is correct. To adjust the concentration of
cells, touch the up or down arrow button one time to adjust the plasma pump flow rate:
• To decrease the concentration of cells, touch the up arrow button.
• To increase the concentration of cells, touch the down arrow button.
4
View the collect port again and re-evaluate the concentration of the cells. If the concentration needs further
adjustment, touch the up or down arrow button again.
5
If the collection phase control is System, allow the system to initiate a collection phase when the RBC
detector detects target cells in the collect line.
6
Continue to monitor the concentration of cells flowing through the collect port, and adjust the
concentration again, if necessary.
CMNC, PMN collection, and depletion procedures
1
Go to the collection status screen.
2
Look through the view port to view the concentration of cells flowing through the collect port. Once the
interface is in the correct position, you should see swirls of cells flowing through the collect port. If the AIM
system is enabled, the image of the collect port on the screen should also show the swirls.
3
Examine the collect line where it exits the centrifuge to ensure the color in the line is correct. To adjust the
color, touch the up or down arrow button one time to adjust the plasma pump flow rate:
• If the color is too light, decrease the collection preference by touching the down arrow button. This
increases the concentration of cells that flow through the collect port.
• If the color is too dark, increase the collection preference by touching the up arrow button. This decreases
the concentration of cells that flow through the collect port.
4
Examine the collect line again and re-evaluate the color. If the color needs further adjustment, touch the up
or down arrow button again.
5
Continue to monitor the collect line and adjust the color again, if necessary.
SpectraOptiaOpsManual.book Page 220 Thursday, May 17, 2018 11:24 AM