tecan Infinite 200 PRO, Instructions For Use Manual

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2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 7
5.7.2 Absorbance Ratio Mode ..................................................................... 102
5.8 Multiple Reads per Well ................................................................... 103
5.8.1 MRW Type ......................................................................................... 103
5.8.2 MRW Size .......................................................................................... 104
5.8.3 MRW Border ...................................................................................... 104
5.8.4 Result Display in MS Excel ................................................................ 106
5.8.5 Miscellaneous Software Features of MRW ........................................ 106
5.9 Optimizing Luminescence Measurements ..................................... 107
5.9.1 Integration Time ................................................................................. 107
5.9.2 Light Level Attenuation ....................................................................... 107
5.10 Optimizing AlphaScreen/AlphaLISA Measurements ..................... 108
5.10.1 Instrument Parameters ....................................................................... 108
5.11 Measurements with Injectors .......................................................... 110
5.11.1 Priming and Washing of the Infinite 200 PRO .................................... 110
5.11.2 Washing ............................................................................................. 114
5.11.3 Before Starting a Measurement with Injectors .................................... 118
5.11.4 Injector Modes (i-control) .................................................................... 118
5.12 Blanking Measurements .................................................................. 123
5.13 Cuvette Measurements .................................................................... 124
5.13.1 Cuvette Strip ...................................................................................... 124
5.13.2 Cuvette Movements ........................................................................... 124
5.13.3 i-control Cuvette Examples ................................................................ 125
5.14 i-control Examples ........................................................................... 129
5.15 Finishing a Measurement Session .................................................. 133
5.15.1 Disconnecting the Instrument ............................................................. 133
5.15.2 Instrument Shut Down ........................................................................ 133
6. Instrument Features ..................................................................................... 135
6.1 Introduction ...................................................................................... 135
6.2 Instrument Specifications ............................................................... 136
6.3 Fluorescence Intensity and Time Resolved Fluorescence (TRF). 138
6.3.1 Definition of the Detection Limit .......................................................... 139
6.3.2 Fluorescein (Fluorescence Intensity) Top .......................................... 139
6.3.3 Fluorescein (Fluorescence Intensity) Bottom ..................................... 139
6.3.4 Europium (Time Resolved Fluorescence) .......................................... 139
6.4 Fluorescence Polarization (FP) ....................................................... 140
6.5 Absorbance ...................................................................................... 141
6.6 Glow Type Luminescence ............................................................... 142
6.6.1 ATP Glow Luminescence ................................................................... 142
6.7 Flash Type Luminescence ............................................................... 143
6.8 Dual Color Luminescence (e.g. BRET) ........................................... 144
6.9 AlphaScreen/AlphaLISA .................................................................. 144
6.10 “On the Fly” Measurements ............................................................ 145