7. Quality Control
156
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
7.2
Specifications - Passed/Failed Criteria
Note
All specifications are subject to change without prior notification.
The following table gives an overview of the passed/failed criteria for the
specification test of the Infinite 200 PRO.
Specification Cuvette (Infinite M200
PRO only)
Passed/Failed Criteria
Absorbance Accuracy
0 –
2 OD: ≤ ± 1 % + 10 mOD
2 –
3 OD: ≤ ± 2.5 %
Absorbance Baseline Flatness (1 sigma)
< ± 10 mOD
Specification
Passed/Failed Criteria
Fluorescence Top Sensitivity
< 20 pM Fluorescein
Fluorescence Top Uniformity
< 3 % CV
Fluorescence Top Precision
< 2 % CV
Fluorescence Bottom Sensitivity
Standard: 100 pM
Enhanced:
Infinite M200 PRO: 45 pM
Infinite F200 PRO: 35 pM
Fluorescence Bottom Uniformity
< 3 % CV
Fluorescence Bottom Precision
< 2 % CV
Time Resolved Fluorescence Sensitivity
(Infinite F200 PRO only)
< 150 fM (with 510 dichroic)
< 3 pM (with 50% mirror)
Time Resolved Fluorescence Precision
(Infinite F200 PRO only)
< 2 % CV
FP Precision (Infinite F200 PRO only)
< 5 mP
Luminescence Sensitivity Glow Type
< 3 fmol/well
Luminescence Sensitivity Flash Type
< 80 amol/well
Absorbance Accuracy
0 –
2 OD: ≤ ± 1 % + 10 mOD
2 –
3 OD: ≤ ± 2.5 %
Absorbance Baseline Flatness
(1 sigma)
< ± 10 mOD
Absorbance Wavelength Accuracy
≤ ± 1.5 nm
λ
> 315 nm;
≤ ± 0.8 nm
λ
≤ 315 nm
AlphaScreen Detection Limit
(Infinite F200 PRO only)
0.2 µg/ml Omnibeads