5. Operating the Instrument
102
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
5.7
Optimizing Absorbance Measurements
5.7.1
Measurement Parameters
Flash Settings
On the fly measurements with 1 flash (read) per well are possible for all plate
types; however, measurement precision at low light levels depends on the
reading time during which a fluorescence signal can be received.
Note
Increase the number of flashes (reads) per well until noise of BLANK
wells does not further improve, or until measurement time per well
becomes unacceptable.
Settle Time
A settle time before measuring a well may be set (critical for absorbance
measurements). Due to the stop and go motion of the plate carrier the meniscus
of the dispensed liquid may still vibrate while signal is integrated. This can give
rise to fluctuations of the measured values. The effect has been observed in wells
of 96-well plates and larger wells.
5.7.2
Absorbance Ratio Mode
Ratio Mode
Using the “
Standard
”-tab in i-control up to 4 labels may be measured well-wise.
This measurement mode is called ‘ratio mode’. Be aware that no ‘ratio’
calculation is performed after this measurement. The Excel result sheet shows
the raw data. Further calculations have to be performed by the user.
Using the “
Applications
”-tab in i-control together with the NanoQuant Plate, the
raw data for “Quantifying Nucleic Acids” and “Labeling Efficiency” are all
automatically calculated for concentration or ratio-calculation by Excel software.
The values can be used for further calculation if preferred.
Wavelength (Infinite M200 PRO)/Filter (Infinite F200 PRO) Switch Time
The
Infinite F200 PRO
can switch between two neighboring filters within 250 ms.
The Infinite M200 PRO can switch between two wavelengths in 150 ms.
For conditions, see 5.5.3 FI Ratio Mode.
