7. Quality Control
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
165
7.3.3
Time Resolved Fluorescence
For the Infinite 200 PRO with the option ‘Fluorescence Top’, the following tests
may be performed to prove the specifications:
•
Sensitivity
•
Precision/Repeatability
These test instructions are valid for the Infinite F200 PRO.
Sensitivity
Perform the following measurement to determine the sensitivity:
Measurement Parameters:
Parameter
Setting
Reading Mode
Fluorescence Top
Ex Wavelength
Infinite F200 PRO: 340 (35) nm
Infinite M200 PRO: 340 nm
Em Wavelength
Infinite F200 PRO: 612 (10) nm
Infinite M200 PRO: 617 nm
Number of flashes
25
Integration Time
400
Lag Time
100
Settle Time
0
Gain
Optimal
Plate Type
GRE96fw
Plate Layout:
Pipette 200 µl of 1 nM Europium solution or the blank solution (enhancement
solution) into the appropriate wells according to the plate layout:
<>
1
2
3
4
5
...
A
1 nM
E
ur
o
pi
um
B
la
n
k
B
la
n
k
B
la
n
k
B
la
n
k
B
C
D
E
F
G
H
