2. General Description
40
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
Luminescence Optics
In luminescence measurement mode, the Infinite 200 PRO uses fixed microplate
position and a moveable luminescence measurement head (see
Optical System Luminescence). The plate thickness is defined by selecting the
corresponding plate type in the software (see i-control Instructions for Use).
Fiber
A glass fiber guides the light from the sample to the detection unit. The fiber is
designed to measure from 6-well up to 384-well plates.
Filter Wheel
A filter wheel with 6 filter positions in front of the PMT window is switched to the
required luminescence channel. The sensitivity of the detection system makes it
necessary to attenuate high luminescence light levels; therefore, the filter wheel
can also switch a neutral density filter across the selected fiber exit.
Filter Wheel Position
Filter
Position 1
Lumi Green*
Position 2
Lumi Magenta*
Position 3
OD2 neutral density filter
Position 4
No attenuation
Position 5
Blue 1**
Position 6
Green 1**
*
recommended for the BRET
2
assay and the ChromaGlo - Luciferase Assay
** recommended for the BRET assay
The OD2 neutral density filter serves to attenuate high light levels by a factor of
100 (corresponding to 2 OD absorbance). The resulting values are automatically
scaled to counts per second and displayed accordingly in the software results
output.
See Figure 16 to Figure 19 for transmission spectra of luminescence filters.
Figure 16: Transmission spectrum of filter ‘Lumi Magenta’
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Wavelength [nm]
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