5. Operating the Instrument
112
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
Priming Procedure (Hardware Button):
Priming can also be performed without using the software. Priming parameters
can be stored on the injector by clicking
Save as Default
on the
Prime
tab of the
Injector Maintenance
dialog box of the i-control software (in the
Settings
menu,
click
Injectors...
and the
Injector Maintenance
dialog box appears). Press the
Prime/Wash button on the injector box to start the priming sequence using the
default parameters, (see Figure 43: Injector-box with injector in ‘service position’,
page 110). The injector must be connected the instrument and the instrument
must be switched on. Start the prime procedure by pressing the Prime/Wash
button for less than 3 seconds.
Visually inspect the syringes for air bubbles. Any bubbles should be removed
after priming to ensure good injection performance.
After a successful priming procedure, reinsert the injector into the instrument.
Close the lid of the pump module completely before starting a measurement. The
injectors are now ready to use.
When starting a measurement with the actions ‘injection’ or ‘dispense’, 5 µl of
liquid are dispensed into a disposable container on the plate carrier before
starting ‘injection’ or ‘dispense’. This initial dispense step makes sure that the
injection/dispense conditions are equal for each well.
CAUTION
CLOSE THE LID OF THE PUMP MODULE (INJECTOR BOX)
COMPLETELY BEFORE STARTING A MEASUREMENT.
Reagent Backflush
The dead volume of the injection system (injector needles, syringes, valves and
tubing) is approximately 100 µl for each syringe after the backflush procedure has
been performed. The function of backflush is to return any unused reagent to the
reservoir bottles.
The injection speed can be adjusted via the software to allow for good mixing of
reagents. The optimal injection speed depends on the assay parameters, such as
viscosity of fluids, the plate format and the measuring behavior of the liquids.
Reagent backflush allows reagents in the tubing system to be pumped back into
storage bottles. This action can be performed optionally prior to washing the
injector system to minimize the dead volume.
Before performing the
Backflush
procedure:
1.
Remove the injector carrier from the instrument and insert the injector carrier
into the service position of the injector box.
2.
Insert the feeding tubing into the appropriate storage bottle.
STOP
