2. General Description
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
33
Mirror Selection - Fluorescence Top (Infinite F200 PRO only)
The Infinite F200 PRO is equipped with a mirror carrier, which houses a 50%
mirror as well as a 510 dichroic mirror or, alternatively, a specific dichroic for
AlphaScreen/AlphaLISA measurements (see 2.5.8 AlphaScreen/AlphaLISA
System (Infinite F200 PRO only)).
The advantage of the 50% mirror is that is works with any pair of excitation and
emission wavelengths. However, 50% of excitation light that is directed into the
sample and, subsequently, 50% of the emission light coming out of the sample
are lost.
Dichroic mirrors are wavelength dependent and are designed to reflect a certain
range of wavelengths almost entirely. Dichroic mirrors exhibit a high reflection of
excitation light and a high transmission of emission light and usually give a better
signal-to-noise ratio compared to 50% mirrors.
Available for plate formats up to 384 wells.
Note:
A dichroic mirror needs to match the selected fluorescence excitation
and emission wavelengths.
Mirror Type
Reflection (Excitation)
Transmission (Emission)
50% mirror
230 – 900 nm
230 – 900 nm
510 dichroic (e.g. fluorescein)
320 – 500 nm
520 – 780 nm
650 dichroic (AlphaScreen/AlphaLISA)
665 – 700 nm
540 – 635 nm
Note:
The dichroic mirror used for AlphaScreen/AlphaLISA cannot be used
for standard fluorescence measurements. It is selected automatically
for Alpha measurements.
According to the wavelengths defined in the measurement script, the dichroic
mirror is selected automatically if both excitation and emission wavelength match
the specified range of that mirror. If either the excitation or the emission
wavelength does not match the ranges of the dichroic mirror, the 50% mirror is
chosen automatically for the measurement.
Fluorescence Optics Bottom
Flash light enters the optical system by being focused through a slit and then
through the excitation filter. The excitation bottom fiber guides the light to the
bottom optics probe, which consists of an elliptical mirror which focuses the light
through the bottom of the microplate into the well. The emitted light is focused
onto the excitation bottom fiber, which guides the light over a mirror through the
emission filter to the fluorescence detection system.
