5. Operating the Instrument
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
93
5.5.3
FI Ratio Mode
Ratio Mode
Up to 4 labels may be measured well-wise. This measurement mode is called
‘ratio mode’. Be aware that no ‘ratio’ calculation is performed after this
measurement. The Excel result sheet shows the raw data. Further calculations
have to be performed by the user.
Filter Switch Time (Infinite F200 PRO)/Wavelength Switch Time (Infinite M200
PRO)
The Infinite F200 PRO can switch between two filters within 250 ms in case that
the selected labels are measured with the same gain. Otherwise, the switching
time is 400 ms. In this case the high voltage level at the PMT needs to be
changed. The high voltage applied to the PMT needs some time to stabilize.
The Infinite M200 PRO can switch between two wavelengths within 150 ms in
case that the selected labels are measured with the same gain and no order
sorting (OS) switching point is involved (see Table 1:for switching points).
Otherwise, the switching time is 400 ms. In this case the high voltage level at the
PMT needs to be changed. The high voltage applied to the PMT needs some
time to stabilize. The OS filter wheel needs to be moved.
Excitation
Wavelength
Emission
Wavelength
OSF Switching Point 1
316 nm
401 nm
OSF Switching Point 2
386 nm
621 nm
OSF Switching Point 3
561 nm
-
Table 1: OSF (Order Sorting Filter) Switching Points (Infinite M200 PRO)
Example:
Fura-2: This application involves a filter/wavelength switch between 340 and
380 nm on the excitation side. The emission is measured at about 510 nm. The
excitation filter/wavelength switch does not include an OS switch; therefore the
switch is possible within 150 ms on an Infinite M200 PRO
and 250 ms on an
Infinite F200 PRO.
