5. Operating the Instrument
94
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
5.5.4
Optimal Read
(FI Bottom Enhanced measurements only)
"Optimal Read" is an automated function available for fluorescence bottom
enhanced measurements in order to achieve maximal well illumination. Multiple,
spatially separated spots are arrayed to cover the whole well area. The total
number of individual measurement spots per well is optimized for plate formats
from 6 to 96 wells (see table below). 384-well plates are optimally illuminated by a
single-spot read.
Plate
Pattern
Spots
Plate
384-well
“Optimal Read” function not available
96-well
Circle
4
96-well
48-well
Circle (filled)
12
48-well
24-well
Circle (filled)
21
24-well
12-well
Circle (filled)
37
12-well
6-well
Circle (filled)
76
6-well
Table 2:
“Optimal Read” spot patterns in different plate formats
Changing the total number of flashes per well (1-100) will result in the automatic
adjustment of the number of flashes per spot, giving the user the possibility to
obtain representative results in each well.
The total number of flashes is automatically distributed over all measured spots.
A minor imprecision occurs if an entered flash number is not divisible without a
remainder by the default number of spots for the used plate format. In this case
the next possible flash distribution that is integrally divisible by the number of
spots per well is calculated, e.g. a measurement with a total of 25-28 flashes in a
96-well plate (4 single spots) is performed with 7 flashes per spot, whereas a total
flash number of 29 results in 8 flashes per spot.
