2. General Description
46
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
Emission Filters
The
Infinite F200 PRO
is equipped with two dedicated emission filters for
AlphaScreen and AlphaLISA measurements, respectively. Depending on the
experimental setup, the user may select either the AlphaScreen or AlphaLISA
emission filter to optimize the signal in the desired wavelength range. The
transmission characteristics of both filters are summarized in figure 23 and figure
24.
Figure 23: Transmission spectrum of filter ‘AlphaScreen’.
Figure 24: Transmission spectrum of filter ‘AlphaLISA’.
Note
The installed Alpha excitation and emission filters are optimized for
AlphaScreen/AlphaLISA measurements and should not be used for
fluorescence measurements.
Note
The dichroic mirror used for AlphaScreen/AlphaLISA cannot be used for
standard fluorescence measurements. It is selected automatically for
Alpha measurements.
PMT Detector
AlphaScreen/AlphaLISA signals are recorded using an ultrasensitive
photomultiplier tube (PMT). Adjusting the PMT gain enables measurement of a
wide range of concentrations with lower or higher signals.
For AlphaScreen and AlphaLISA measurements it recommended to calculate the
gain from a well containing the highest signal/concentration using the ‘calculated
from well’ function. For fastest-possible measurement speed, the gain may be
determined in a pre-measurement of the well with the highest signal and then set
manually. For details see Section 5.5.1 Instrument Parameters.
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