2. General Description
42
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
2.5.7
Cuvette Port (Infinite M200 PRO)
The Infinite M200 PRO may be optionally equipped with a cuvette port for
absorbance measurements.
For absorbance measurements with the cuvette port of Infinite M200 PRO a
similar optical path is used as for fluorescence excitation.
The absorbance system consists of:
1.
light source
2.
excitation monochromator
3.
absorbance cuvette measurement module
4.
absorbance microplate module
Condenser type optics focus the light through the excitation filter and then
through the entrance slit to the excitation monochromator. A fiber bundle then
guides the light from the excitation monochromator to the absorbance cuvette
optics, which focuses the light through the cuvette. The absorbance cuvette
measurement module is located right after the cuvette port. A silicon photo diode
measures the light being transmitted through the sample. Before measurement of
the cuvette, a reference measurement against air is performed with the cuvette
port moved out of the light beam.
Figure 20: Optical System of the absorbance module of Infinite M200 PRO including the
cuvette port The figure also shows the light path of the absorbance microplate module (5).
For details of the light source (1) and the excitation monochromator (2), please
refer to chapter 2.4.1 Fluorescence/A) Fluorescence Intensity (FI).
