7. Quality Control
174
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
7.3.11 AlphaScreen/AlphaLISA (Infinite F200 PRO only)
For the Infinite F200 PRO with the option ‘AlphaScreen/AlphaLISA’ the following
test may be performed to prove the sensitivity specification. These test
instructions are valid only for the Infinite F200 PRO.
Sensitivity
Perform the following measurement to determine the detection limit for
AlphaScreen Omnibeads.
Material:
AlphaScreen Omnibeads (#6760626D, PerkinElmer)
Greiner 384-well plate, flat bottom, white
Phosphate-buffered saline (PBS)
10 µl p tips
100 µl p tips
Omnibeads dilution series:
Dilute the Omnibeads stock solution 1:500 in PBS by adding 3 µl of the stock
solution (5 mg/ml) to 1497 µl PBS (yielding a solution of 10 µg/ml).
Prepare 12 further dilutions in 1:2 steps by pipetting 750 µl of the previous
dilution step to 750 µl PBS.
Plate layout:
Pipette 100 µl of each dilution into 5 replicate wells of the microplate (as shown
below). Use 100 µl/well PBS for the Blank wells.
Use a fresh tip for each concentration and take care NOT to contaminate
the blank with any Omnibeads dilution!
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
A
10,000 ng/ml
B
5,000 ng/ml
C
2,500 ng/ml
D
1,250 ng/ml
E
625 ng/ml
F
312 ng/ml
G
156 ng/ml
H
78 ng/ml
I
39 ng/ml
J
20 ng/ml
K
10 ng/ml
L
5 ng/ml
M
2.5 ng/ml
N
Blank (PBS only)
O
P
