5. Operating the Instrument
78
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
5.4
Defining Filter Slides (Infinite F200 PRO only)
5.4.1
About Filters
Fluorescence Filters
The optical filters (bandpass style) in a filter slide are specially designed for
fluorescence measurements. The spectral rejection and the bandwidth of the
fluorescence filters are optimized for achieving excellent sensitivity.
Contact TECAN for filters other than those supplied on the delivered filter slides.
Absorbance Filters
Bandpass filters, which are commonly used in microplate readers for absorbance
measurements, usually have a bandwidth of 10 nm. Therefore it is not
recommended to use fluorescence filters for absorbance measurements because
the bandwidth (FWHM) is usually larger than 10 nm. This could cause a bright
value error or low OD values when measuring dyes with narrow peaks.
5.4.2
Filter Slide and Filter Orientation
Filter Slide
The Infinite F200 PRO filter slide consists of an excitation and an emission part.
The filter slide enables the user to work with four independent excitation/emission
filter pairs, which can be defined on positions 1 to 4. The information about the
inserted filters is saved on the integrated microchip.
Figure 36: Infinite F200 PRO: Filter slide
