4. Gas Control Module (Enhanced)
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
69
11.
Press ‘
I/O’
key in selected gas control mode to switch on the gas flow
(regulation). The plate carrier compartment should be closed during
equilibration until the target gas level is reached.
12.
Insert a microplate into the instrument and start gas regulation.
During gas flow regulation in CO2 Mode, O2 Mode and DUAL Mode, the
LED is blinking. In Manual Mode the LED is blinking when the gas valve is
open.
13.
When the measurement is finished, press the ‘I/O’ key to stop gas
regulation.
14.
Switch off the gas control module with the ON/OFF switch on the rear
panel.
15.
Close the valves of the gas cylinder(s), remove the tubes from the GCM
and let out any remaining gas from the tubing.
IMPORTANT
MAKE SURE TO APPLY A SUITABLE GAS-PERMEABLE ADHESIVE
FOIL, TAPE, OR COVER TO THE MICROPLATE. SEALING THE PLATE
ALLOWS FOR VENTING CULTURES WHILE SIMULTANEOUSLY
ACTING AS A BARRIER TO REDUCE EVAPORATION DURING GAS
SUPPLY.
WARNING
TREAT BIO-HAZARDOUS MATERIAL ACCORDING TO APPLICABLE
SAFETY STANDARDS AND REGULATIONS.
IMPORTANT
ALWAYS INCLUDE APPROPRIATE POSITIVE AND/OR NEGATIVE
CONTROLS IN YOUR ASSAY TO REFLECT EFFECTS ON CELL
VIABILITY DURING INCUBATION.
IMPORTANT
ENSURE THAT A SUFFICIENT SUPPLY OF CO
2
OR N
2
IS PROVIDED
DURING INCUBATION. RUNNING OUT OF GAS OR FAILURE OF GAS
SUPPLY MAY NEGATIVELY EFFECT OR HARM YOUR CELL
APPLICATION.
Acoustic and Visual Alarm
If the target concentration is not reached after 20 minutes upon initial activation of
a mode or when it deviates for more than 10 minutes during operation (i.e. with a
deviation (> +/- 20%) an acoustic signal will warn you and the display will start
blinking. This will help you to recognize, for example, when the gas supply has
run out (tank is empty).
If power is lost – the valves of the gas control module will close automatically.
