6. Instrument Features
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
139
6.3.1
Definition of the Detection Limit
The detection limit is the fluorophore concentration where the background-
subtracted signal equals 3 times the standard deviation of the background noise.
When selecting 1 flash per well, the plate carrier does not stop at the
measurement position. Using more flashes per well may improve the detection
limit, but the total measurement time will be longer.
6.3.2
Fluorescein (Fluorescence Intensity) Top
Plate Type (number of wells)
96
Dispensed Volume [µl]
200
Flashes (Reads) per Well
25
Fluorescein Detection Limit [pM]
< 20 pM
Uniformity at 25 nM Fluorescein
< 3 % CV
Reproducibility at 25 nM Fluorescein
< 2 %
6.3.3
Fluorescein (Fluorescence Intensity) Bottom
Plate Type (number of wells)
96
Dispensed Volume [µl]
200
Flashes (Reads) per Well
25
Fluorescein Detection Limit [pM]
Standard: 100 pM
Enhanced:
Infinite M200 PRO: 45 pM
Infinite F200 PRO: 35 pM
Uniformity at 25 nM Fluorescein
< 3 % CV
Reproducibility at 25 nM Fluorescein
< 2 %
6.3.4
Europium (Time Resolved Fluorescence)
Plate Type (number of wells)
96
Dispensed Volume [µl]
200
Flashes (Reads) per Well
25
Europium Detection Limit
(Infinite F200 PRO)
< 150 fM
Europium Detection Limit
(Infinite M200 PRO)
< 5 pM (Typical value)
