5. Operating the Instrument
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
131
Example 3: Measurement of Ca
2+
sensitive probes – Fura-2
Plate
Select an appropriate plate type. For fluorescence measurements, black
microplates are recommended.
For this example, a black 96 well plate was selected.
Part of the plate
(Not shown), can be optionally selected if only part of the plate should be
processed
Well
Mandatory for measurements with ‘injection’
Kinetic Cycle
Select the number of necessary cycles
Kinetic condition
This strip allows actions to be performed once in a kinetic run at a certain
cycle. The intended injection strip below it is only processed once at the
selected cycle.
Injection
Injector A injects 20 µl with speed 200 µl/sec., refill mode: not selectable;
injection is performed at cycle 5 (defined by kinetic condition strip)
Fluorescence
Intensity (1)
Select the appropriate parameters for the first label: Excitation wavelength:
380 nm, Emission wavelength: 510 nm; number of flashes: 25; integration
time: 40; gain: manual
Fluorescence
intensity (2)
Select the appropriate parameters for the second label: Excitation
wavelength: 340 nm, Emission wavelength: 510 nm; number of flashes:
25; integration time: 40; gain: manual
Move Plate
Plate is moved out after finishing all wells
