5. Operating the Instrument
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
109
PMT Properties
The gain for fluorescence intensity is selectable from 1 – 255. The performance
of the PMT depends on the supply voltage. The Infinite 200 PRO PMTs are
specified from 300 to 1250 V. The relationship between the gain settings of the
Infinite 200 PRO and the voltage supply is described in Equation 1. The intended
use of the Infinite 200 PRO PMT is therefore specified for gain settings from 60 to
255. Gain settings below 60 are possible, but the performance of the PMT is not
specified for voltage supply < 300 V. Tecan therefore does not take responsibility
for measurement results of Infinite 200 PRO when using gain settings below 60.
Equation 4:
V
1250
*
255
Gain
U
=
Where U is the voltage, Gain is the selected gain setting, 255 is the maximum
possible gain and 1250 V is the maximum voltage supply of the PMT.
Example:
A gain of 100 corresponds to a voltage supply of 490 V:
Equation 5:
V
490
1250
*
255
100
U
=
=
