5. Operating the Instrument
88
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
Equation 1:
V
1250
*
255
Gain
U
=
Where U is the voltage, Gain is the selected gain setting, 255 is the maximum
possible gain and 1250 V is the maximum voltage supply of the PMT.
Example:
A gain of 100 corresponds to a voltage supply of 490 V:
Equation 2:
V
490
1250
*
255
100
U
=
=
5.5.2
Z-Optimization (FI Top measurements with the Infinite
M200 PRO only)
A useful feature of the
Infinite M200 PRO
is the z-optimization procedure.
Z-Optimization is only available for FI Top measurements with the Infinite M200
PRO). For a particular assay, this procedure should be performed once to
determine the optimum working distance between the sample in the plate and the
fluorescence optics.
The z-position can be determined as follows:
(1)
‘Manual’
:
When using the option ‘manual’, a numeric z-position value can be entered
in the measurement stripe. The default manual z-position is 20000 µm.
(2)
‘Calculated from well’
:
When using the option ‘calculated from well’, the
Infinite M200 PRO
will
automatically identify the z-position of maximum signal in the selected well
for further measurements.
(3)
‘Same as’
for multi-labeling measurements:
When using the option ‘same as’, the
Infinite M200 PRO
will automatically
use the same z-position as for a previously defined label.
E.g. in a script with 2 FI Top labels named as Label 1 and Label 2 the z-
position of Label 1 can also be used for Label 2 by selecting the option
‘Same as = Label 1’.
(4)
‘Instrument’
Z-Position’:
When using the ‘Z-position’ function in the instrument menu, the user can
determine the appropriate z-position from a graphical plot that shows the
well(s) used for z-positioning. The selected value is applied for further
measurements.
