7. Quality Control
160
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
Precision
Perform the following measurement to determine the precision/reproducibility:
Measurement Parameters:
Parameter
Setting
Reading Mode
Fluorescence Top
Ex Wavelength
Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Em Wavelength
Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
Number of flashes
25
Integration Time
40
Settle Time
0
Gain
Optimal
Plate Type
GRE96fb
Part of the Plate
A1
Kinetic
20 Cycles
Interval Time
Minimal
Plate Layout:
Pipette 200 µl of 1 nM Fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
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1
2
3
4
5
6
7
8
9
10
11
12
A
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM
F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
B
C
D
E
F
G
H
Material/Reagents:
1 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate black
200 µl P tips
Calculation of Precision:
1
wellA
1
wellA
mean
100
*
stdev
%)
CV
(
ecision
Pr
=
mean
wellA1
Average RFU value of well A1 over the 20 kinetic
stdev
wellA1
Standard deviation of RFU values of Well A1 over the 20 cycles
The result of the formula determines the Precision in % CV.
