7. Quality Control
158
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
Plate Layout:
Pipette 200 µl of 1 nM Fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
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1
2
3
4
5
6
7
8
9
10
11
12
A
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM
F
lu
or
es
c
ei
n
B
la
n
k
B
C
D
E
F
G
H
Material/Reagents:
1 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate black
200 µl P tips
Calculation of Detection Limit (Sensitivity):
(
)
B
B
F
F
Stdev
*
3
*
mean
mean
ion
Concentrat
imit
DetectionL
−
=
Concentration
F
Concentration of the fluorophore in pM units
mean
F
Average RFU value of wells filled with fluorophore
mean
B
Average RFU value of wells filled with blank
stdev
B
Standard deviation of RFU values of wells filled with blank
The result of the formula 'Detection Limit' determines the sensitivity in pM units.
